University of Valencia
Department of Physiology, Laboratorio de Investigación en Células Endoteliales
INCLIVA Biomedical Research Institute
Carlos Hermenegildo
Laboratorio de Investigación en Células Endoteliales (L.In.CE) research group is a multidisciplinary team of expert researchers in Vascular and Endocrine Physiology, Molecular Biology, Biochemistry, Cell Biology and Reproductive Biology.
Main lines of research:
Gender differences in cardiovascular area
Vascular effects of sex hormones
Identification of new hormone-regulated signalling pathways in endothelium
Interaction of sex hormones with pro-atherogenic factors
miRNA profile in vascular cells under estrogenic exposure
Expertise
We perform studies on gene and protein expression, vasoactive substance production measurement and functional approaches on cardiovascular pathophysiology at three levels:
Cultured endothelial cells
Experimental animal models (mainly senescence accelerated mouse and experimental menopause)
Human samples
Techniques:
Molecular biology techniques: PCR, RT-PCR, microarrays…
Biochemical techniques: western blot, immunofluorescence, ELISA, HPLC…
Cell biology techniques: primary cultures of human endothelial and smooth muscle cells, flow cytometry…
Vascular physiology techniques: vascular reactivity
Interested in collaborating on
To discover and to analyse the disease mechanisms at the vascular level.
To study the endothelial and vascular repercussion of the diseases and treatments by means of studying the miRNA, mRNA and protein expression profiles and the endothelial functionality.
To discover the implication of sex and gender differences on the vascular consequences of diseases and therapeutic interventions.
To identify biomarkers of cardiovascular diseases.
To share our collaboration with clinicians and our access to large cohorts of controls and patients with cardiovascular diseases
Selected publications on ncRNA and cardiovascular disease
miRNA as a New Regulatory Mechanism of Estrogen Vascular Action
Pérez-Cremades D, Mompeón A, Vidal-Gómez X, Hermenegildo C, Novella S. Int J Mol Sci. 2018, 19(2). pii: E473.
MicroRNA as Crucial Regulators of Gene Expression in Estradiol-Treated Human Endothelial Cells
Vidal-Gómez X, Pérez-Cremades D, Mompeón A, Dantas AP, Novella S, Hermenegildo C. Cell Physiol Biochem. 2018, 45(5):1878-1892.
